β 1 integrin cd29 monoclonal antibody  (Bio X Cell)

Journal: The Journal of Clinical Investigation

Article Title: Integrin-mediated mTOR signaling drives TGF- β overactivity and myxomatous mitral valve degeneration in hypomorphic fibrillin-1 mice

doi: 10.1172/JCI183558

Figure Lengend Snippet: ( A ) Representative IF and number of CD45 + cells ( n = 4) and mean fluorescence density (AU) for p-S6 ( n = 3–4) and p-Smad2 ( n = 3–4) in 5-week-old WT and mgR mice treated with either IgG2a control or Itgb1 mAb for 1 week starting at 4 weeks of age. Scale bar: 25 μm. ( B ) Schema depicting serologic neutralization experimental design in which mgR mice were treated with Itgb1 mAb for 8 weeks starting at 4 weeks of age with mitral valves analyzed at 12 weeks of age. ( C ) Incidence of MR ( n = 10) and ( D ) morphometric analysis of anterior mitral valve leaflet area ( n = 5). ( E ) Measurement of maximal leaflet thickness ( n = 4–6) and ( F ) mean fluorescence density (AU) for p-S6 ( n = 3) in 12-week-old WT, α5/2, mgR, mgR + Itgb1 MAb, and α5/2 mgR mice. ( G ) Representative Movat pentachrome staining of long-axis sections of mitral valve leaflets and ( H ) representative IF of p-S6 in 12-week-old WT, α5/2, mgR, mgR + Itgb1 MAb, and α5/2 mgR mice. Scale bars: 100 μm. Data are represented as individual values with mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 by ( D – F ) 1-way or ( A ) 2-way ANOVA or ( C ) Fisher’s exact test.

Article Snippet: Animals were treated with (a) rapamycin (Calbiochem) at 2 mg/kg/d i.p. every other day (q.o.d.) versus DMSO vehicle alone, (b) β 1 integrin (CD29) monoclonal antibody (BioXCell BE0232) at 4 mg/kg/d i.p. q.o.d. versus IgG2a isotype control (BioXCell BE0089), and (c) TGF-β mAb (BioXCell BE0057) at 250 μg i.p. q.o.d. versus IgG1 isotype control (BioXCell #BE0083) for various durations as described, including a final dose 6 hours before euthanasia.

Techniques: Fluorescence, Control, Neutralization, Staining, IF-P

Journal: The Journal of Clinical Investigation

Article Title: Integrin-mediated mTOR signaling drives TGF- β overactivity and myxomatous mitral valve degeneration in hypomorphic fibrillin-1 mice

doi: 10.1172/JCI183558

Figure Lengend Snippet: Representative IF and mean fluorescence density (AU) in human normal mitral valves and MVP specimens for ( A ) p-Smad2 and p-S6 ( n = 8), and ( B ) CD45 ( n = 8–9) and β 1 integrin (ITGB1) ( n = 8–10). Scale bars: 50 μm. ( C ) Uniform Manifold Approximation and Projection (UMAP) plots of single-cell RNA-Seq with annotation of primary cell types, demonstrating proportional differences between mitral valves from normal and MVP specimen ( n = 4–6). Volcano plot showcasing DEGs of ( D ) fibroblasts and ( E ) macrophages from normal and MVP valves, with selected DEGs labeled. Genes highlighted in red are upregulated in MVP, while those in blue are upregulated in normal valves. ( F ) Chord diagram illustrating the strength of receptor-ligand signaling interactions among macrophages and fibroblasts in MVP versus normal as reference; *integrins identified as receptors. Data are represented as individual values with mean ± SEM; *** P < 0.001, **** P < 0.0001 by unpaired t test.

Article Snippet: Animals were treated with (a) rapamycin (Calbiochem) at 2 mg/kg/d i.p. every other day (q.o.d.) versus DMSO vehicle alone, (b) β 1 integrin (CD29) monoclonal antibody (BioXCell BE0232) at 4 mg/kg/d i.p. q.o.d. versus IgG2a isotype control (BioXCell BE0089), and (c) TGF-β mAb (BioXCell BE0057) at 250 μg i.p. q.o.d. versus IgG1 isotype control (BioXCell #BE0083) for various durations as described, including a final dose 6 hours before euthanasia.

Techniques: Fluorescence, RNA Sequencing, Labeling

Journal: PLoS ONE

Article Title: Development of a Curved, Stratified, In Vitro Model to Assess Ocular Biocompatibility

doi: 10.1371/journal.pone.0096448

Figure Lengend Snippet: Following exposure to stimuli after 6 or 24 hours, the curved, stratified cultures of corneal epithelial cells were digested with trypsin and EDTA to form the adherent cell population. CD49c and CD29 expression was measured by flow cytometry and is expressed as a ratio relative to its expression on cells incubated with no lens or BAK (control). n = 3 to 5, *significantly different from PBS-soaked lens, p<0.05. BA PBS: PBS-soaked balafilcon A lens.

Article Snippet: Monoclonal antibodies to β 1 integrin (CD29) and α 3 integrin (CD49c) were fluorescein isothiocyanate (FITC) and R-phycoerythrin conjugated, respectively and were purchased from Becton Dickinson (Mountain View, CA, USA).

Techniques: Expressing, Flow Cytometry, Incubation, Control

Journal: PLoS ONE

Article Title: Development of a Curved, Stratified, In Vitro Model to Assess Ocular Biocompatibility

doi: 10.1371/journal.pone.0096448

Figure Lengend Snippet: Effect of BAK exposure and PBS-soaked lenses on cell integrin expression in a curved, stratified culture of corneal epithelial cells after 6 and 24 hours.

Article Snippet: Monoclonal antibodies to β 1 integrin (CD29) and α 3 integrin (CD49c) were fluorescein isothiocyanate (FITC) and R-phycoerythrin conjugated, respectively and were purchased from Becton Dickinson (Mountain View, CA, USA).

Techniques: Expressing

Journal: Molecular Vision

Article Title: Effect of contact lens material on cytotoxicity potential of multipurpose solutions using human corneal epithelial cells

doi:

Figure Lengend Snippet: HCEC β 1 (integrin CD29) expression after 24 h contact with Lotrafilcon A (LA), Balafilcon A (BA), Lotrafilcon B (LB), Galyfilcon A (GA), and Comfilcon A (CA) lenses soaked in various MPS. Integrin expression was measured by flow cytometry and is expressed as a percentage relative to cells grown in the absence of a lens. n=3 to 4, * significantly different from cells grown in the absence of lens and PBS-soaked lens (p<0.045), # significantly different from respective buffer control soaked lens (p≤0.04). Complete - Complete Moisture Plus; OFX - Opti-Free Express; ReNu - ReNu MultiPlus; Solo - SoloCare Aqua; BBS - borate buffer saline; PBS - phosphate buffer saline.

Article Snippet: Monoclonal antibodies to β 1 integrin (CD29; Immunotech-Coulter, Marseilles, France) was fluorescein isothiocyanate (FITC) conjugates.

Techniques: Expressing, Flow Cytometry, Control, Saline

Journal: Molecular Vision

Article Title: Effect of contact lens material on cytotoxicity potential of multipurpose solutions using human corneal epithelial cells

doi:

Figure Lengend Snippet: HCEC α 3 (integrin CD49c) expression after 24 h contact with Lotrafilcon A (LA), Balafilcon A (BA), Lotrafilcon B (LB), Galyfilcon A (GA), and Comfilcon A (CA) lenses soaked in various MPS. Integrin expression was measured by flow cytometry and is expressed as a percentage relative to cells grown in the absence of a lens. n=4 to 5, * significantly different from cells grown in the absence of lens and PBS-soaked lens (p<0.04), # significantly different from its respective buffer control soaked lens (p≤0.04). Complete - Complete Moisture Plus; OFX - Opti-Free Express; ReNu - ReNu MultiPlus; Solo -SoloCare Aqua; BBS - borate buffer saline; PBS - phosphate buffer saline.

Article Snippet: Monoclonal antibodies to β 1 integrin (CD29; Immunotech-Coulter, Marseilles, France) was fluorescein isothiocyanate (FITC) conjugates.

Techniques: Expressing, Flow Cytometry, Control, Saline

Journal: Molecular Vision

Article Title: Effect of contact lens material on cytotoxicity potential of multipurpose solutions using human corneal epithelial cells

doi:

Figure Lengend Snippet: Effect of MPS concentration on cell viability, β 1 (integrin CD29) and α 3 (integrin CD49c) expression after 24 h incubation.

Article Snippet: Monoclonal antibodies to β 1 integrin (CD29; Immunotech-Coulter, Marseilles, France) was fluorescein isothiocyanate (FITC) conjugates.

Techniques: Concentration Assay, Expressing, Incubation

Journal:

Article Title: Inducement of mitogen-activated protein kinases in frozen shoulders

doi: 10.1007/s00776-008-1295-6

Figure Lengend Snippet: Immunohistochemical examination of synovial tissues in a frozen shoulder. A H&E stain. B CD29 β1-integrin. C ERK. D p38. E JNK. F NF-κB. (×200)

Article Snippet: Tissue sections were blocked for 10 min in PBS containing 20% rabbit serum, followed by incubation overnight at 4°C with the following antibodies: anti-human phospho-p38 MAPK (Tyr180/Tyr182) mouse monoclonal antibody (1: 500) (3216S; Cell Signaling Technology, Danvers, MA, USA); anti-human phospho-p44/42 MAPK (Tyr202/Tyr204, ERK1/ERK2) mouse monoclonal antibody (1: 500) (9106S; Cell Signaling Technology); anti-human phosphor-JNK (1: 500) (G7) (sc-6254; Santa Cruz Biotechnology, Santa Cruz, CA, USA); anti-human NF-κB p50 rabbit polyclonal antibody (1: 200) (sc-7178; Santa Cruz Biotechnology), anti-TNFα mouse monoclonal antibody (1: 1000) (Biogenesis, Pool, UK), anti-human CD29 (β 1 -integrin) mouse monoclonal antibody (1: 350) (NCL-CD29; Novocastra, Newcastle upon Tyne, UK), MMP-3(1: 100) (5980-0311; Biogenesis, Mill Creek, WA, USA), IL-6 (1: 6000) (109-401-310; Rockland Immunochemicals, Rockland, ME, USA); CD56 (1: 1000) (NCL-CD56; Novocastra); CD68 (1: 1000) (M0814; Dako, Carpenteria, CA, USA); S-100 (1: 5000) (Z0311; Dako); and VEGF (1: 800) (05-443; Upstate/Millipore, Billerica, MA, USA).

Techniques: Immunohistochemical staining, Staining

Journal:

Article Title: Inducement of mitogen-activated protein kinases in frozen shoulders

doi: 10.1007/s00776-008-1295-6

Figure Lengend Snippet: Results for immunohistochemical staining of synovial tissue in frozen shoulders

Article Snippet: Tissue sections were blocked for 10 min in PBS containing 20% rabbit serum, followed by incubation overnight at 4°C with the following antibodies: anti-human phospho-p38 MAPK (Tyr180/Tyr182) mouse monoclonal antibody (1: 500) (3216S; Cell Signaling Technology, Danvers, MA, USA); anti-human phospho-p44/42 MAPK (Tyr202/Tyr204, ERK1/ERK2) mouse monoclonal antibody (1: 500) (9106S; Cell Signaling Technology); anti-human phosphor-JNK (1: 500) (G7) (sc-6254; Santa Cruz Biotechnology, Santa Cruz, CA, USA); anti-human NF-κB p50 rabbit polyclonal antibody (1: 200) (sc-7178; Santa Cruz Biotechnology), anti-TNFα mouse monoclonal antibody (1: 1000) (Biogenesis, Pool, UK), anti-human CD29 (β 1 -integrin) mouse monoclonal antibody (1: 350) (NCL-CD29; Novocastra, Newcastle upon Tyne, UK), MMP-3(1: 100) (5980-0311; Biogenesis, Mill Creek, WA, USA), IL-6 (1: 6000) (109-401-310; Rockland Immunochemicals, Rockland, ME, USA); CD56 (1: 1000) (NCL-CD56; Novocastra); CD68 (1: 1000) (M0814; Dako, Carpenteria, CA, USA); S-100 (1: 5000) (Z0311; Dako); and VEGF (1: 800) (05-443; Upstate/Millipore, Billerica, MA, USA).

Techniques: Immunohistochemical staining, Staining